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KMID : 0900919990230020133
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Korean journal of Animal Reproduction
1999 Volume.23 No. 2 p.133 ~ p.139
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In Vitro/In Vivo Development of Vitrified Immature Mouse Oocytes
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Yi B.-K.
Kim E.-Y.
Nam H.-K.
Lee K.-S.
Yoon S.-H.
Park S.-P.
Lim J.-H.
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Abstract
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This study was carried out to investigate in vitro/in vivo development of vitrified-thawed immature mouse oocytes. Immature mouse oocytes were vitrified with EFS40 (40% ethylene glycol, 18% ficoll and 0.5 M sucrose). Thawed oocytes were matured for 16 hr in vitro. Matured oocytes with the first polar body were fertilized with the concentration of 1~2times10^{6} mell of epididymal sperm. After fertilization, cleavage (geq 2-cell) and in vitro/in vivo development rates were examined. pi Ie results were summarized as follows: in vitro maturation rate of immature mouse oocytes in vitrified-thawed group was similar to that in exposed group (67.5%) and control (66.3%), but cleavage rate of vitrified-thawed oocytes (64.9 %) and blastocyst formation rate (59.0%) were significantly different compared to those of exposed group (83.7 and 74.7%) and control (90.7 and 83.7%) (p＜0.05). However, when the blastocysts derived from immature mouse oocytes vitrified-thawed were transferred to pseudopregnant mouse, total implantation (31.3%) was slightly lower than that in control (40.8%), but live fetus formation rate (66.7%)was slightly higher than that in control (58.1%), there was not significantly different. Therefore, when the blastocyts produced in vitro were transferred into recipients, although the development in vitro of oocytes vitrified-thawed was decreased, live fetus formation rate was similar to that of control group. The present results indicate that immature mouse oocytes can be frozen successfully by vitrification with EFS40.
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KEYWORD
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Mouse immature oocytes, Vitrification, EFS40, In vivo development
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